Fibronectin Type III Domain-containing Protein 5, Fibronectin Type III Repeat-containing Protein 2, FNDC5
Irisin is a recently described exercise-induced hormone secreted by skeletal muscle in mice and humans. Irisin promotes brown adipocyte recruitment in white fat and improves systemic metabolism by increasing energy expenditure. Irisin is cleaved from the type I membrane protein FNDC5.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: detection Western Blot: 1:1000 Optimal dilutions to be determined by researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to Irisin.
Form
Supplied as a liquid in PBS, 0.02% sodium azide, 10% glycerol. Labeled with Biotin.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes Irisin. Species Crossreactivity: human, mouse, rat and monkey. Detects endogenous mouse and human Irisin
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