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045854 Mouse Anti-B220/CD45R (Cluster of Differentiation 45, CD45R) (FITC)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Mouse
Conjugate
FITC
Isotype
IgG2a
Clone Number
RA3-6B2
Grade
Affinity Purified
Applications
FC
Crossreactivity
Mo
Gene ID
19264
Shipping Temp
Blue Ice
Storage Temp
-20°C

B220 is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells (1‑3). The level of B220 antigen expression on the B cell lineage is developmentally regulated (2‑4) and this antibody is commonly used as a B cell marker.

045854 has been reported to inhibit in vivo B cell responses (5, 6).
Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 10ul labels 10e6 cells using mouse splenocytes Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Abelson mouse leukemia virus-induced pre-B tumor cells
Form
Supplied as a liquid in saline, <0.5% BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Specificity
Recognizes mouse B220/CD45R
References
1. Coffman, R.L. (1982) Immuno. Rev. 69:5.|2. Hardy, R.R. et al. (1991) J. Exp. Med. 173:1213.|3. Hathcock, K.S. et al. (1992) J. Immunol. 149:2286.|4. Allman, D.M. et al. (1992) J. Immunol. 149:2533.|5. Asensi, V.K. et al. (1989) Immunology 68:204.|6. Domiati-Saad, R. et al. (1993) J. Immunol. 151:5936.
USBio References
No references available
Conjugates
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