Polyethylene glycols (PEG) are non-toxic, uncharged, hydrophilic long chain polymers with a big exclusion volume in aqueous solution. The covalent attachment of PEG is commonly employed to modify proteins and drugs that can increase their bioavailability and reduce immunogenicity and proteolytic degradation.
052322 is a rat monoclonal IgM antibody that displays high affinity to both short and long PEG molecules. It displays specificity for the repeating subunits present in PEG and can bind a broad range of PEG molecules. Use of CHAPS detergent instead of Tween-20 is highly recommended in all washing buffers.
Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilutions
ELISA: 5ug/ml of Polyethylene Glycol (PEG) (10K-PEG, 3K-PEG, and 2K-mPEG) were detected Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
PEG-conjugated to E.coli beta-glucuronidase.
Form
Supplied as a liquid in PBS, 0.04% sodium azide.
Purity
Purified by size-exclusion chromatography.
Specificity
Recognizes the the repeating subunits present in PEG. Displays high affinity to both short and long PEG molecules.
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