CDK2 a serine/threonine protein kinase, is a member of a highly conserved family of protein kinases that regulate the eukaryotic cell cycle. The protein kinase is homologous to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. For activity, it requires association with a cyclins which act as the positive regulatory subunit. It forms complex with cyclins A, E, D1, and D3. Cyclin E- CDK2 kinase is active in the G1 and S phases of the cell cycle and is important for the progression from G1 to S phase. The levels of Cyclin A- CDK2 are maximal at the G1/S transition and both CDK2 and Cyclin A associate with DNA in the initiation complex during replication.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
AA Sequence
MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Full length human CDK2, aa1-298 (NP_001789.2).
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human CDK2.
TDS
NCBI
UniProt
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