CDK2 a serine/threonine protein kinase, is a member of a highly conserved family of protein kinases that regulate the eukaryotic cell cycle. The protein kinase is homologous to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. For activity, it requires association with a cyclins which act as the positive regulatory subunit. It forms complex with cyclins A, E, D1, and D3. Cyclin E- CDK2 kinase is active in the G1 and S phases of the cell cycle and is important for the progression from G1 to S phase. The levels of Cyclin A- CDK2 are maximal at the G1/S transition and both CDK2 and Cyclin A associate with DNA in the initiation complex during replication.

Suitable for use in Western Blot. Other applications not tested.

Optimal dilutions to be determined by the researcher.

MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Full length human CDK2, aa1-298 (NP_001789.2).

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.

Purified by Protein A affinity chromatography.

Recognizes human CDK2.