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124963-FITC Mouse Anti-CHN1 (N-chimaerin, A-chimaerin, Alpha-chimerin, N-chimerin, NC, Rho GTPase-activating Protein 2, ARHGAP2, CHN) (FITC)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
FITC
Isotype
IgG2a,k
Clone Number
3A3
Grade
Affinity Purified
Applications
FLISA WB
Crossreactivity
Hu Mo Rt
Accession #
BC011393, AAH11393
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
BSA Free

GTPase-activating protein for p21-rac and a phorbol ester receptor. Involved in the assembly of neuronal locomotor circuits as a direct effector of EPHA4 in axon guidance.

Applications
Suitable for use in FLISA and Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
AA Sequence
QTRNFRLYYDGKHFVGEKRFESIHDLVTDGLITLYIETKAAEYIAKMTINPIYEHVGYTTLNREPAYKKHMPVLKETHDERDSTGQDGVSEKRLTSLVRRATLKENEQIP
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Partial recombinant corresponding to aa91-200 from human CHN1 (AAH11393) with GST tag. MW of the GST tag alone is 26kD.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human CHN1. Species Crossreactivity: mouse and rat.
References
1. Tsc2-Rheb signaling regulates EphA-mediated axon guidance. Nie D, Di Nardo A, Han JM, Baharanyi H, Kramvis I, Huynh T, Dabora S, Codeluppi S, Pandolfi PP, Pasquale EB, Sahin M.Nat Neurosci. 2010 Jan 10. [Epub ahead of print]
USBio References
No references available
Conjugates
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