Calcitonin gene-related peptide (CGRP) is present in C-cells of the thyroid and in central and peripheral nerves. CGRP has several important physiologic roles: it is a potent vasodilator, and can affect the force and rate of heart beat; it can modulate acetylcholine receptor function at the neuromuscular junction; it has been demonstrated to block tolerance to morphine; and it can modulate antigen presentation in Langerhans cells in the skin.
Applications
Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunofluorescence: 1:100-1:200 (Fluorescein), 1:1000-1:2000 (Streptavidin/HRP) Immunohistochemistry: 10um cryostats. Fixative: 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4; 500 mL over ~20-30min. Post Fixation: 1.5h at 4°C in 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic rat alpha-CGRP coupled to bovine thyroglobulin (BTg) with glutaraldehyde.
Form
Supplied as a lyophilized powder, 0.09% sodium azide. Reconstitute with 100ul ddH2O.
Specificity
Recognizes rat CGRP. Species Crossreactivity: human.
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