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138514-AF-APC Rabbit Anti-CD3e (CD 3E, CD3 epsilon, CD3 TCR complex, CD3E, CD3e antigen epsilon polypeptide (TiT3 complex), T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE, TiT3 complex) (APC)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
3003, P07766
Conjugate
APC
Isotype
IgG
Grade
Affinity Purified
Applications
FC IF WB
Crossreactivity
Hu Mo
Accession #
NP_000724
Gene ID
916
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Applications
Suitable for use in Western Blot, Flow Cytometry and Immunofluorescence. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Positive Control
Jurkat cells. Tonsil and lymph node
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to aa156-168 of human CD3e (ERPPPVPNPDYEP). Cellular Localization: Cell surface and cytoplamic
Form
Supplied as a liquid in PBS. No preservative added. BSA-Free. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human CD3e. Recognizes the e-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kD. Species Crossreactivity: mouse. Species sequence homology: elephant, gibbon, guinea pig, hamster, equine, rat, monkey, mouse, orangutan, porine, rabbit, sheep, ape, feline, chicken, chimpanzee, bovine, canine, frog, opossum.
References
1. Cibull ML et. al. Histopathology, 1989, 15(6):599-605. 2. Mason DY et. al. Journal of Clinical Pathology, 1989, 42(11):1194-200.
USBio References
No references available
Conjugates
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