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303221 Rabbit Anti-CD86 (Cluster of Differentiation 86, Activation B7 2 Antigen, Activation B7-2 Antigen 3, Activation B7-2 Antigen, Activation B72 Antigen, B Lymphocyte Activation Antigen B7 2, B Lymphocyte Activation Antigen B72, B-lymphocyte Activation Antigen B7-2 2, B-lymphocyte Activation Antigen B7-2, B7 2, B7 2 Antigen, B7, B7-2, B7.2, B70, B72 Antigen, BU63, CD 86, CD28 Antigen Ligand 2 2, CD28 Antigen Ligand 2, Cd28l2, CD28LG2, CD86 Antigen (CD28 Antigen Ligand 2, B7-2 Antigen) 1, 2, CD86 Antigen (CD28 Antigen Ligand 2, B7-2 Antigen), CD86 Antigen, CD86 Molecule, CD86_HUMAN, CLS1, CTLA 4 Counter Receptor B7 2, CTLA 4 Counter Receptor B7.2, CTLA-4 Counter-receptor B7.2 2, 3, CTLA-4 Counter-receptor B7.2, CTLA4 Counter Receptor B72, Early T-cell Costimulatory Molecule 1, ETC-1, FUN 1, FUN-1, FUN1, LAB72, Ly-58, Ly58, MB7, MB7-2, Membrane Glycoprotein, T Lymphocyte Activation Antigen CD86, T-lymphocyte Activation Antigen CD86, TS/A-2)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P42081
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Cluster of Differentiation 86 (also known as CD86 and B7-2) is a protein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to human CD86 expressed in E. coli. (Position: A24-F329). Species sequence homology: mouse (50%)
Form
Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human CD86.
References
1. Chen C, Gault A, Shen L, Nabavi N (May 1994). "Molecular cloning and expression of early T cell costimulatory molecule-1 and its characterization as B7-2 molecule". J. Immunol. 152(10): 4929–36. 2. Reeves, R. H., Patch, D., Sharpe, A. H., Borriello, F., Freeman, G. J., Edelhoff, S., Disteche, C. The costimulatory genes Cd80 and Cd86 are linked on mouse chromosome 16 and human chromosome 3. Mammalian Genome 8: 581-582, 1997. 3. Shah, R., Banks, K., Patel, A., Dogra, S., Terrell, R., Powers, P. A., Fenton, C., Dinauer, C. A., Tuttle, R. M., Francis,G. L. Intense expression of the B7-2 antigen presentation coactivator is an unfavorable prognostic indicator for differentiated thyroid carcinoma of children and adolescents. J. Clin. Endocr. Metab. 87: 4391-4397, 2002.
USBio References
No references available
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