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368662 Mouse Anti-CD274 (B7-H, B7H1, PDL1, PD-L1, PDCD1L1, PDCD1LG1)

Specifications
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG2b
Clone Number
7D2A10
Grade
Purified
Applications
E FC WB
Crossreactivity
Hu
Gene ID
29126
Shipping Temp
Blue Ice
Storage Temp
-20°C

This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants.

Applications
Suitable for use in Flow Cytometry, ELISA, Western Blot. Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:200-1:400 ELISA: 1:10,000 Western Blot: 1:500-1:2000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein fragment corresponding to aa24-153 of human CD274 expressed in E. coli.
Form
Supplied as a liquid in PBS, 0.05% sodium azide
Purity
Purified
Specificity
Recognizes human CD274.
Conjugates
Pricing
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