USBio Logo

397015 Mouse Anti-CD32B (FcRIIB, Fcgr2b, Ly-17) (FITC)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Mouse
Swiss Prot
P08101
Conjugate
FITC
Isotype
IgG1
Clone Number
AT130-5
Grade
Affinity Purified
Applications
FC
Crossreactivity
Mo
Gene ID
14130
Shipping Temp
Blue Ice
Storage Temp
-20°C

CD32B (FcRIIB) is a low affinity receptor for IgG1, IgG2a and IgG2b type antibodies. Unlike other FcRs, the intracellular domain of CD32B contains a inhibitory signalling motif (ITIM) that can antagonize signal transduction mediated through other activatory receptors. FcRIIB is expressed on all myeloid populations and on B-cells.

Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Flow Cytometry: Neat 1:10 Optimal dilutions to be determined by the researcher.
Hybridoma
NS-1 myeloma cells with spleen cells from mice.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Fusion protein corresponding to mouse CD32, rat CD4.
Form
Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Purity
Purified by Protein A affinity chromatography from tissue culture supernatant.
Specificity
Recognizes mouse CD32.
References
1. White, A. et al. (2011) Interaction with FcγRIIB is critical for the agonistic activity of anti-CD40 monoclonal antibody. J Immunol. 187: 1754-1763|2. Wiiliams, E.L. et al. (2012) Development and characterisation of monoclonal antibodies specific for the murine inhibitory FcγRIIB (CD32B). Eur J Immunol. 42: 2109-20
USBio References
No references available
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
Contact Us

Visit our technical library or contact our support staff to answer your questions.

Telephone:
1-800-520-3011

Library | Contact

Distributors

For customers outside of the United States, please use one of our many distributors.

View Distributors

Payment Methods

We accept the following payment methods as well as pay-by-invoice.

MasterCard Visa PayPal
© 2023-2024 United States Biological - All Rights Reserved