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A0901-80F-ML490 Mouse Anti-ADP Ribosylation Factor 1 (ARF1, DJ133P16.1) (MaxLight 490)

Specifications
References
Brand
MaxLight™
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P84077
Conjugate
MaxLight™490
Isotype
IgG2a
Clone Number
9H195 (3F1)
Grade
Affinity Purified
Applications
FLISA IHC WB
Crossreactivity
Hu Mo Rt
Accession #
NM_001024226.1
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.

ARF1 (ADP-ribosylation factor 1) is a member of the ARF family of small G proteins (guanine nucleotide-binding proteins). It is a component of Golgi-derived non-clathrin coated vesicles. Arf and other related small GTPases are an important part of the membrane traffic with both exocytic and endocytic pathways. ARF1 helps regulate the binding and releasing of coat proteins on membranes to form transport vesicles. It is also known to play a role in vessicular trafficking of phospholipase D.
Applications
Suitable for use in FLISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:2000 Immunohistochemistry (paraffin): 1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to the C-terminal amino acid sequence of human ARF1.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human ADP Ribosylation Factor 1. Species Crossreactivity: mouse and rat
References
1. Beck, R. et al. (2008). Proc Natl Acad Sci. 105(33):11731-11736. 2. Krauss,M. et al. (2008) J. Biol. Chem. 283(41):27717-23.
USBio References
No references available
Conjugates
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