Applications
Suitable for use in Immunohistochemistry and Immunofluorescence. Other applications not tested.
Recommended Dilution
Immunofluorescence: 1:200 Immunohistochemistry (PAP or ABC): 1:500 Optimal dilutions to be determined by the researcher.
AA Sequence
Ser-Ser-Arg-Arg-Cys-Val-Arg-Leu-His-Glu-Ser-Cys-Leu-Gly-Gln-Gln-Val-Pro-Cys-Cys-Asp-Pro-Cys-Ala-Thr-Cys-Tyr-Cys-Arg-Phe-Phe-Asn-Ala-Phe-Cys-Tyr-Cys-Arg-Lys-Leu-Gly-Thr-Ala-Met-Asn-Pro-Cys-Ser-Arg-Thr-NH2 (Contains 5 disulfide bridges. Precise positions are undetermined)
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding aa82-132 of human AGRP.
Form
Supplied as a lyophilized powder. Reconstitute with sterile dH2O.
Specificity
Recognizes human AGRP. Species Crossreactivity: Human Agouti-Related Protein Form C-NH2. % Cross-reactivity with Peptides: Agouti-Related Protein (83-132)-NH2 (Human): 100%; Agouti-Related Protein Form C-NH2 (Human): 100%; Leptin (Human): 0%; Orexin A (Human, Mouse, Rat): 0%; Orexin B (Human): 0%; Neuropeptide Y (Human, Rat): 0%; a-MSH: 0%; MCH (Human, Mouse, Rat): 0%; CGRP (Rat): 0%; CGRP (Human): 0%