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A2295-07E Rabbit Anti-Angiotensin II Type 1 Receptor (AT1)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Rat
Isotype
IgG
Grade
Affinity Purified
Applications
E
Crossreactivity
Ca Hu Mo Po Rb Rt Sh
Shipping Temp
Blue Ice
Storage Temp
-20°C

Angiotensin II interacts with two types of G-protein coupled membrane receptors, AT1 (type 1) and AT2 (type 2). Major cardiovascular effects appear to be mediated through AT1. AT1 has three isoforms (rat AT1A 359aa; AT1B/AT III, 359aa; and AT1C, 177aa). Rat AT1 receptors are predicted to contain seven transmembrane domains. The N-terminus is predicted to be extracellular. The C-terminus is inside the membrane (cytoplasmic). The binding of angiotensin II with AT1 receptors activate a phosphatidylinositol-calcium second messenger system. AT1 receptors are expressed in the liver, kidney, aorta, lung, uterus, ovary, spleen, heart, adrenal and vascular smooth muscle. AT2 gene ( chromosome x) encodes 363 aa protein. It is highly expressed in myometrium with lower levels in adrenal and fallopian tube. It is also expressed at high levels in fetal kidney and intestine.

Applications
Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested.
Recommended Dilutions
Western Blot: 1-10ug/ml (ECL). Detects ~45kD band. ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. Optimal dilutions to be determined by the researcher.
Control Peptide
A2295-07G: Angiotensin II Type 1 Receptor, Rat (AT1)
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic 10aa peptide sequence mapping near the N-terminus of rat AT II (KLH). Cellular Localization: Extracellular. Species sequence homology: mouse, human, porcine, sheep, canine, chimpanzee and rabbit: 100%.
Form
Supplied as a liquid in PBS, 0.1% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes rat Angiotensin II type 1 Receptor. Sequence is the same in all AT II receptor isoforms (Type 1a, 1b and 1c). No sequence homology with other G-protein coupled receptors.
References
1. Murphy, T.J., et al., Nature 351: 233 (1991). 2. Iwai, N., et al., BBRC 177: 299 (1991). 3. Langford, K., et al., BBRC 183: 1025 (1992). 4. Sasamura, H., et al., BBRC 185: 253 (1992). 5. Yoshida, H., et al., BBRC 186: 1042: (1992). 6. Mauzy, C.A., et al., BBRC 183: 8 (1992). 7. Bergsma, D.J., et al., BBRC 183: 989 (1992). 8. Konishi, H., et al., BBRC 199: 467 (1992). 9. Iwai, N., et al., FEBS Lett. 298: 257 (1992). 10. Sandberg, K., et al., BBRC 183: 1090 (1992). 11. Elton, T.S., et al., BBRC 184: 1067 (1992). 12. Hahn, A.W., et al., BBRC 192: 1260 (1993).
USBio References
No references available
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