Suitable for use in ELISA. Other applications not tested.

Peptide ELISA: 1:1,000. Western Blot: 1ug/ml. A 26-28kD band in Human Brain is detected. reliminary experiments gave an approx 26-28kD band in Human Brain (Amygdala, Cerebellum, Hippocampus) lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 49.1kDa according to NP_065843.3. The 26-28kD band was successfully blocked by incubation with the immunizing peptide. Optimal dilutions to be determined by the researcher.

May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Corresponding peptide is available for Peptide Blocking studies. See A3596-01-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.

A synthetic peptide from the C-terminus of aa-(RTRNSYIKWLDQN).

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

Purified by affinity chromatography.

Recognizes human arylacetamide deacetylase-like 1. Species Crossreactivity: mouse, canine.