The human calbindin D28k (CALB1) is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27kD protein induced by vitamin D in the duodenum of the chick. Parmentier et al. (1991) mapped the CALB1 gene to 8q21.3-q22.1 by in situ hybridization.
Application
Suitable for use in Western Blot, Immunoprecipitation, and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 0.5-1ug/ml Immunohistochemistry (paraffin): 1-2ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH20. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Bovine kidney calbindin-D.
Form
Supplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute with 1.2% sodium acetate, PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes rat Calbindin-D.
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