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C2381-03K Mouse Anti-CD29 (Integrin beta-1, Fibronectin Receptor Subunit beta, VLA-4 Subunit beta, ITGB1, FNRB, MDF2, MSK12)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1,k
Clone Number
TS2/16
Grade
Affinity Purified
Applications
FC IF IHC IP
Crossreactivity
Bo Ca Eq Hu Mk
Shipping Temp
Blue Ice
Storage Temp
-20°C

CD29 is a 130kD single chain type I glycoprotein, known as integrin β1, VLA-β chain, or gpIIa. It is broadly expressed on a majority of hematopoietic and non-hematopoietic cells, including leukocytes (although at low level on granulocytes), platelets, fibroblasts, endothelial cells, epithelial cells, and mast cells. CD29 is a member of the integrin family. It is non-covalently associated with integrin α1-α6 chains to form VLA-1 to VLA-6 molecules, respectively. Integrins which include CD29 bind to several cell surface (e.g. VCAM-1, MadCAM-1) and extracellular matrix molecules. CD29 acts as a fibronectin receptor and is involved in a variety of cell-cell and cell-matrix interactions.

Applications
Suitable for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human CD29.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human CD29. Species Crossreactivity: African Green Monkey, baboon, bovine, Cynomolgus Monkey, canine, equine and Rhesus Monkey.
References
1. Schlossman, S., et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 2. Gutierrez-Lopez, M., et al. 2003. J. Biol. Chem. 278:208. 3. Hemler, M.E., et al. 1984. J. Immunol. 132:3011. 4. Sanchez-Aparicio, P., et al. 1994. J. Cell Biol. 126:271. 5. Frank, N.Y., et al. 2005. Cancer Res. 65:4320. 6. Murga, M., et al. 2005. Blood 105:1992. 7. Porter, J.C. and Hogg, N., 1997. J. Cell Biol. 138:1437. 8. Conway, R. E., et al. 2006. Mol. Cell. Biol. 26:5310. 9. Hemler, M. 1990. Annu. Rev. Immunol. 8:365. 10. Hynes, R. 1992. Cell 69:11.
USBio References
No references available
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