Suitable for use in ELISA, Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested.

Western Blot: 0.1-0.2ug/ml Flow Cytometry: This antibody was tested in flow cytometry using mouse splenocytes. Dilute this antibody to 50ug/ml and add 10 μL of the diluted solution to 1-2.5 x 10e5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as anti-goat IgG conjugated to a fluorochrome. Immunohistochemistry: 5-15ug/mL Optimal dilutions should be determined by each laboratory for each application.

Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

NS0-derived rmCD31 extracellular domain

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with 500ul sterile PBS.

Purified by immunoaffinity chromatography.

Selected for its ability to recognize mouse CD31 in the applications listed. In direct ELISAs and Western Blots, this antibody shows ~10% crossreactivity with rhCD31 and rpCD31.