Suitable for use in Flow Cytometry and ELISA. Other applications not tested.

Flow Cytometry: 80ul at 5ug/ml labels 5x10e5 cells. Optimal dilutions to be determined by the researcher.

1.Five x 10e5 cultured Raji cells were washed and incubated 45 minutes on ice with 80ul C2432-01F at 5ug/ml. 2. Cells were then washed twice, incubated with secondary reagent. 3. Cells were then washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 1.81 log10 fluorescent units when compared to a Mouse IgM/FITC negative control at a similar concentration. 4. Binding was blocked when pre incubated 10 minutes with 20ul of 0.5mg/ml C2432-01E.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Human CD80 (B7-1) Ig Fusion Protein.

Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.

Purified by Protein A affinity chromatography.

Recognizes human CD80.