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C9097-40F Mouse Anti-Cytokeratin 7

Specifications
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P35900
Isotype
IgG1
Clone Number
2Q1133 (OV-TL 12/30)
Grade
Purified
Applications
FC IC IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40kD and 68kD and isoelectric pH between 4.9-7.8. The individual cytokeratin polypeptides are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Applications
Suitable for use in Western Blot, Flow Cytometry, Immunocytochemistry and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:100-1:1000 Immunohistochemistry (Paraffin): 1:100-1:200, pretreated with 0.1% pronase/0.1% trypsin in PBS, 0.5% pepsin in 0.01N HCl or microwave pretreatment. Immunohistochemistry (Frozen): Fixed with methanol Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Cytoskeletal preparation of OTN-11 ovarian carcinoma cell line.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified.
Specificity
Recognizes human Cytokeratin 7. Reacts with specific glandular-type epithelia and most carcinomas derived from them.
Conjugates
Pricing
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