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D1550-01D-Biotin Mouse Anti-DC-SIGN (Dendritic Cell-specific ICAM3 grabbing Non-integrin, C Type Lectin Domain Family 4 Member L, CLEC4L, CD209, CD209 Antigen, CDSIGN, Dendritic Cell-specific ICAM-3-grabbing Non-integrin 1, DC SIGN1, DC-SIGN1, HIV GP120 Binding Protein, MGC129965) (Biotin)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
Biotin
Isotype
IgG1
Clone Number
MR-1
Grade
Affinity Purified
Applications
FC IF
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
BSA Free
CD209 Molecule

CD209 is involved in dendritic cell (DC) migration and the initial interaction between DC's and naive T lymphocytes. CD209 also binds HIV-1 gp120 and plays a key role in the dissemination of HIV-1 by DC's.

Applications
Suitable for use in Flow Cytometry and Immunofluorescence. Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Immature myeloid monocyte-derived dendritic cells (MDDCs).
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human DC-specific ICAM-3 grabbing nonintegrin (DC-SIGN), a 45kD C-type lectin that binds ICAM-3 that is also known as CD209. Reported to partially block the functional activity of DC-SIGN(2).
References
1. Relloso, M. et al. (2002) DC-SIGN (CD209) expression is IL-4 dependent and is negatively regulated by IFN, TGF-beta, and anti-inflammatory agents. J. Immunol. 168: 2634 - 2643. 2. Melero, I. et al. (2002) An anti-ICAM-2 (CD102) monoclonal antibody induces immune-mediated regressions of transplanted ICAM-2-negative colon carcinomas. Cancer Res. 62: 3167 - 3174
USBio References
No references available
Conjugates
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