Suitable for use in Immunohistochemistry and RIA. Other applications have not been tested.
Recommended Dilution
Immunohistochemistry (frozen): 1:250-1:2500 Radioimmunoassay: 1:5000 Optimal working dilutions to be determined by researcher.
Recommended Secondary Reagents
Sheep anti-Rabbit IgG (HRP)
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Dynorphin B 1-13: Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Gln-Phe-Lys-Val-Val-Thr (porcine) Prodynorphin 228-240.
Form
Supplied as a liquid in PBS, 0.09% sodium azide, 1% BSA.
Purity
Serum
Specificity
The antibody blocks specifically with Dynorphin B 1-13. Cells are found in the paraventricular hypothalamic and the supraoptic nucleus in rat brain of colchicine treated 4% FA perfused tissue. Fibers are found in the lateral hypothalamus of both colchicine and normal rat brain of 4% FA perfused tissue. |Species Crossreactivity: Porcine, rat, syrian hamster, guinea pig and rhesus monkey. Not tested on Human
References
1. Khachaturinan, H., et al., Peptides 6(Suppl.2): 155-166 (1985). 2. Sherman, T.G., et al., J. Neuroscience 8: 3797-3811 (1988). 3. Neal, C.R. & Newman, S.W., J. Comp. Neurology 288: 353-386 (1989).
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