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D9905-04C Goat Anti-DAG1 (Dystroglycan, Dystrophin-associated Glycoprotein 1, Alpha-dystroglycan, alpha-DG)

Specifications
References
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E
Crossreactivity
Bo Ca Hu Mo Rt
Accession #
NP_004384.3
Gene ID
1605
Shipping Temp
Blue Ice
Storage Temp
-20°C

The dystroglycan complex is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:128,000 Western Blot: 0.2-0.6ug/ml, human skeletal muscle lysates observed on ~100kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-HVGKHEYFMHATDK, from human DAG1, at the internal region of the protein (NP_004384.3).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human DAG1. Species sequence homology: Bovine, canine, mouse and rat.
References
1. de Bernabé DB, Inamori K, Yoshida-Moriguchi T, Weydert CJ, Harper HA, Willer T, Henry MD, Campbell KP, Loss of alpha-dystroglycan laminin binding in epithelium-derived cancers is caused by silencing of LARGE. The Journal of biological chemistry 2009 Apr 284(17):11279-84.
USBio References
No references available
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