Fluorescein isothiocyanate (FITC) is a small fluoresent molecule that is commonly conjugated to antibodies for immunofluorescent cell labeling. In conjunction with anti-FITC antibodies, it can also be used as an epitope tag for detection and signal amplification.
Applications
Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
FITC conjugated to KLH
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with horseradish peroxidase (HRP).
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes FITC but not the carrier protein.