Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:500-1:2000 Western Blot: 1:500-1:2000 Immunohistochemistry: 1:20-1:2000 Immunocytochemistry: 1:50-1:5000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Form
Supplied as a liquid in 30mM triethanolamine, pH 7.2, 5mM magnesium chloride, 0.1mM zinc chloride, 1% BSA, Protease/IgG free, 0.05% sodium azide. Labeled with Alkaline Phosphatase (AP).
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human IgA.