Suitable for use in FLISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry and Immunomicroscopy. Other applications not tested.

Immunohistochemistry: 1:20-1:2000 Immunocytochemistry: 1:50-1:5000 Optimal dilutions to be determined by the researcher.

494nm

518nm

7 Moles FITC per Mole Antibody

Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Purified human IgE, epsilon Chain

Supplied as a liquid in 10mM sodium phosphate, 0.15M sodium chloride, pH 7.2, 1% BSA (Protease and IgG free), 0.05% sodium azide. Labeled with Fuorescein Isothiocyanate (FITC).

Purified by affinity chromatography. ≥95% by SDS-PAGE.

Recognizes epsilon chains on human IgE based on IEP. No reactivity is observed with light chains on human immunoglobulins and on non-immunoglobulin human serum proteins.