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J7876-08 Rabbit Anti-Jumonji/ARID Domain Containing Protein 1A (JARID1A, KDM5A, Retinoblastoma Binding Protein 2, RBBP2, RBP2, Retinoblastoma Binding Protein 2 Homolog, RBP2-H1)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P29375
Isotype
IgG
Grade
Affinity Purified
Applications
IP WB
Crossreactivity
Hu Mo
Accession #
NP_005047.1
Gene ID
5927
Shipping Temp
Blue Ice
Storage Temp
-20°C

The protein encoded by this gene is a ubiquitously expressed nuclear protein. It binds directly, with several other proteins, to retinoblastoma protein which regulates cell proliferation. This protein also interacts with rhombotin-2 which functions distinctly in erythropoiesis and in T-cell leukemogenesis. Rhombotin-2 is thought to either directly affect the activity of the encoded protein or may indirectly modulate the functions of the retinoblastoma protein by binding to this protein. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Applications
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Western Blot: 1:2000-1:10,000 Immunoprecipitation: 4-10ug/mg lysate Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
The epitope recognized maps to a region between residue 1640 and 1690 of human Jumonji/ARID Domain-Containing Protein 1A.
Form
Supplied as a liquid in TBS, pH 7.2, 0.1% BSA, 0.09% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Jarid1A/RBP2 protein. |Species Crossreactivity: mouse.
References
1. Liefke R, Oswalkd F, Alvarado C et al. Histone demethylase KDM5A is an integral part of the core Notch-RBP-J repressor complex. Genes Dev 2010 Mar. 2. Ge Z, Li W, Wang N et al. Chromatin remodeling: recruitment of histone demethylase RBP2 by Mad1 for transcriptional repression of a Myc target gene, telomerase reverse trancriptase. FASEB J 2010 Feb. 3. Tsai WW, Nguyen TT, Shi Y et al. p53-targeted LSD1 functions in repression of chromatin structure and transcription in vivo. Mol Cell Biol 2008 Sep
USBio References
No references available
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