Lysosomes are membrane-bound organelles found within the cytoplasm of most cells, which contain hydrolytic enzymes and act as the major compartment for heterophagic and autophagic digestion. Members of the lysosomal-associated membrane protein family (LAMPS) are believed to play an important role in protecting the lysosomal membrane from protease degradation and are involved in lectin-mediated cell adhesion. Evidence shows that CD107b is involved in the lysosomal uptake of cytosolic proteins and the endocytic pathway and human studies have revealed a correlation between the level of surface expression of CD107b on tumor cells and their metastatic potential.

Suitable for use in Western Blot, Electron Microscopy, Immunoprecipitation, Immunofluorescence and Flow Cytometry. Other applications not tested.

Optimal dilutions to be determined by the researcher.

Spleen cells from immunized BALB/c mice were fused with cells of the NS1 myeloma cell line.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

MDCK (Madin-Darby Canine Kidney) cells.

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.

Purified by Protein G affinity chromatography from tissue culture supernatant.

Recognizes canine CD107b, a 95kD heavily-glycosylated type I integral lysosomal glycoprotein. Demonstrates staining pattern consistant with localization to lysosome. Species Crossreactivity: human and mink. Does not react with mouse or rat