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L9190-15D Mouse Anti-Lysosome Associated Membrane Protein 2 (LAMP 2, CD107b, Igp2, Igp110)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Canine
Isotype
IgG1
Clone Number
9A182 (AC17)
Grade
Affinity Purified
Applications
FC IF IP WB
Crossreactivity
Ca Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Lysosomes are membrane-bound organelles found within the cytoplasm of most cells, which contain hydrolytic enzymes and act as the major compartment for heterophagic and autophagic digestion. Members of the lysosomal-associated membrane protein family (LAMPS) are believed to play an important role in protecting the lysosomal membrane from protease degradation and are involved in lectin-mediated cell adhesion. Evidence shows that CD107b is involved in the lysosomal uptake of cytosolic proteins and the endocytic pathway and human studies have revealed a correlation between the level of surface expression of CD107b on tumor cells and their metastatic potential.

Applications
Suitable for use in Western Blot, Electron Microscopy, Immunoprecipitation, Immunofluorescence and Flow Cytometry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:100-1:500 Flow Cytometry: 10ul labels 1x10e6 cells in 100ul. Membrane permeabilization is required for this application. Optimal dilutions to be determined by the researcher.
Hybridoma
Spleen cells from immunized BALB/c mice were fused with cells of the NS1 myeloma cell line.
Recommended Negative Control
I1904-78R: IgG1 Murine Negative Control
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
MDCK (Madin-Darby Canine Kidney) cells.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes canine CD107b, a 95kD heavily-glycosylated type I integral lysosomal glycoprotein. Demonstrates staining pattern consistant with localization to lysosome. Species Crossreactivity: human and mink. Does not react with mouse or rat
References
1. Nabi, I.R., et al., J. Cell Biol. 115: 1573-1584 (1991). 2. Nabi, I.R. and Rodriguez-Boulan, E., Mol. Biol. Cell 4: 627-635 (1993). 3. Tzuu-Shuh, J., et al., Mol. Biol. Cell 11: 287-304 (2000). 4. Ihrke, G., et al., EMBO. J. 22: 6256-6264 (2001). 5. Fukuda, M., J. Biol. Chem. 266: 21,327-21,330 (1991). 6. Pluhar, G.E. et al (2010) Anti-tumor immune response correlates with neurological symptoms in a dog with spontaneous astrocytoma treated by gene and vaccine therapy. Vaccine. 28: 3371-8. 7. Cliffe, S.T. et al. (2009) SLC29A3 gene is mutated in pigmented hypertrichosis with insulin-dependent diabetes mellitus syndrome and interacts with the insulin signaling pathway. Hum Mol Genet. 18: 2257-65.
USBio References
No references available
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