Neurexin 1 (NRXN1) is a transmembrane neuronal protein and member of the neurexin family, which act as cell adhesion molecules and receptors within the vertebrate nervous system. NRXN1 exists in many distinct isoforms, the majority of which encode the longer alpha-neurexins generated using an upstream promoter, whilst fewer encode the shorter beta-neurexins, generated using a downstream promoter. Alpha-neurexins have been shown to bind with high affinity to the neuronal protein neurexophilin, whilst neuroligin 1, enriched in synaptic plasma membranes, has been identified as a splice site-specific ligand for beta-neurexins.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:32000 Western Blot: 0.05-0.1ug/ml Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies
Rabbit Anti Goat IgG (Fc) FITC, HRP
Recommended Useful Reagents
HUMAN CEREBELLUM, LEFT TOTAL PROTEIN LYSATE HUMAN CEREBELLUM, RIGHT TOTAL PROTEIN LYSATE
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Peptide Blocking
Corresponding peptide is available for Peptide Blocking studies. See N2110-04A-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Immunogen
Peptide corresponding to sequence C-KEKQPSSAKSSNKN from the C-Terminal region of human NRXN1. Species sequence homology: mouse, rat, and canine.
Form
Supplied as a liquid in TBS, pH 7.3, 0.02% sodium azide, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes an epitope within the CT region of human Neurexin 1 in cerebellum cell lysates at ~150kD.
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