Suitable for use in ELISA, Western Blot and Immunocytochemistry/ Immunofluorescence. Other applications not tested.

Western Blot: Detects a band at ~65kD. Immunofluorescence (IC): 5ug/ml KO Validated: Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. CACO-2 cells were transfected with Occludin siRNA and reduction of specific signal was observed in immunofluorescence application Optimal dilutions to be determined by the researcher.

T84 cell line (human intestinal epithelium), MDCK cells (canine kidney), Caco-2 cells (human colon adenocarcinoma) and rat liver. Does not appear to detect the most highly phosphorylated form of the occludin protein when tested on Caco-2 cells.

Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

GST fusion protein consisting of the C-terminal region (~150aa) of human occludin.

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide, 50% glycerol. Labeled with Fluorescein isothiocyanate (FITC).

Purified by Protein A affinity chromatography.

Recognizes human Occludin Protein. Species Crossreactivity: mouse, rat and canine.