Osteopontin, also named early T lymphocyte activation protein 1, secreted phosphoprotein, and bone sialoprotein 1, is a secreted acidic phosphorylated glycoprotein originally isolated from bone matrix. It has been shown to bind to different cell types through interactions with various integrins and CD44 variants. It has important functions in bone metabolism and inflammatory processes.
Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 10ul to label 1x10e6 U937 human histiocytic lymphoma cell line Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
Recombinant protein corresponding to Ile17-Asn300 human Osteopontin expressed in NS0 cells
Form
Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein A affinity chromatography from hybridoma culture supernatant
Specificity
Recognizes human Osteopontin. Does not recognize recmbinant mouse Osteopontin
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