RAB8A; RAB8A, member RAS oncogene family; MEL; RAB8; mel transforming oncogene; mel transforming oncogene (RAB8 homolog); mel transforming oncogene (derived from cell line NK14); mel transforming oncogene (derived from cell line NK14)- RAB8 homolog; ras-a
Applications
Suitable for use in ELISA, Western Blot. Other applications not tested.
Recommended Dilution
Peptide ELISA: 1:32,000. Western Blot: 0.1-0.3ug/ml. Approx 25kD band observed in Human Brain (Cerebellum), Kidney and Heart lysates (calculated MW of 23.7kD according to NP_005361.2). Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Peptide sequence C-NQGVKITPDQQKR from the C-Terminal region of RAB8A (NP_005361.2).
Form
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human RAB8A. Species Crossreactivity: canine, bovine.
References
Functional dissection of Rab GTPases involved in primary cilium formation., Yoshimura S, Egerer J, Fuchs E, Haas AK, Barr FA., J Cell Biol. 2007 Jul 30;178(3):363-9. Epub 2007 Jul 23.