4-1 BB Ligand, Recombinant, Human (4-1BBL, CD137L, TNFSF9, Tumor necrosis factor ligand
superfamily member 9)
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| Human 4-1BB Ligand (4-1BBL, CD137L) is a 32kD type II transmembrane protein that belongs to TNF superfamily (TNFSF) of molecules.1-4 The human 4-1BBL cDNA encodes a 254 amino acid (aa) protein that contains a 25aa N-terminal cytoplasmic domain, a 23aa transmembrane segment, and a 206aa C-terminal extracellular region.5 The extracellular domain (ECD) of 4-1BBL has a jelly-roll, -sandwich tertiary structure that is similar to other TNFSF members. There is only one cysteine in the human ECD, and no potential N-linked glycosylation sites. The potential exists, however, for O-linked glycosylation. The human 4-1BBL ECD shares 32% and 35% aa identity with mouse and rat ECD, respectively. In the cytoplasmic domain, human 4-1BBL is 55aa shorter than the equivalent region in rodents. 4-1BBL is expressed by activated B cells, macrophages, dendritic cells, activated Tcells, neurons and astrocytes.2, 3, 6 A 26kD soluble form of 4-1BBL is known to occur in human. Although it is presumably generated by MMP activity, its amino acid size is currently unreported.4 The soluble form is bioactive. Human 4-1BBL signals through both CD137/4-1BB and itself. Its cytoplasmic tail participates in reverse signaling that induces apoptosis in T cells and cytokine secretion (IL-6; TNF- ) by monocytes.7, 8 4-1BBL binding to CD137/4-1BB produces a number of effects. Its seems to play a key role in the T cell recall response. It maintains T cell numbers at the end of a primary response, and induces CD4+ and CD8+ T cells to proliferate and secrete cytokines such as IL-2 and IFN- in CD4+ cells, and IFN- in CD8+ cells.9, 10 | | | Catalog # | 0004-01 | | Source | Recombinant, Human from E. coli | | Endotoxin | 0.1ng/ug (1EU/ug) | | Biological Activity | Determined by the dose-dependent stimulation of IL-8 production by human PBMC (peripheral blood mononuclear cells). The ED50 for this effect is 5-10ng/ml. | | Please Note | Results may vary with PBMC (peripheral blood mononuclear cells) donors. | | Reconstitution | We recommend a quick spin followed by reconstitution in water to | | a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. | | Stability | The Lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted 4-1BBL should be storred in working aliquots at -20°C. | | Country of Origin | USA | | Molecular Weight | 19.5kD | | Source | Recombinant, Human from E. coli | | Purity | 98% by SDS-PAGE and HPLC. | | Form | The sterile filtered solution was lyophilized from 5mM Tris, pH 8.0, 75mM sodium chloride. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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