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Specifications

Clone Host Grade Applications Monoclonal Rat Affinity Purified E B FC

4-1BB Ligand is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF9). It is expressed on activated antigen presenting cells (APCs) and on most T and B lymphoma cell lines. Soluble 4-1BB Ligand can be released from the cell surface following cellular activation via proteolytic cleavage. 4-1BB Ligand exists as a noncovalent homotrimer and interacts with 4-1BB (TNFRSF9) to provide T cell costimulatory signals and dendritic cell activation.
Catalog #	0004-01D
Applications	Suitable for use in Western Blot, Flow Cytometry and ELISA. Other applications not tested.
Recommended Dilution	Western Blot: 1-2ug/ml. The detection limit is ~1ng/lane under non-reducing and reducing conditions.
	Flow Cytometry: 25ug/ml. 10ul of the diluted solution labels 1-2.5 x 10e5 cells in a total reaction volume 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10ul of a 25ug/ml stock solution of a secondary developing reagent such as Gt xRt IgG conjugated to a fluorochrome.
	Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~1.5ng/well.
	Optimal dilutions to be determined by the researcher.
Hybridoma	Fusion of a mouse myeloma with B cells obtained from a rat immunized with purified, Sf 21-derived, recombinant mouse 4-1BB Ligand extracellular domain.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG2b
Clone No	8H117
Host	Rat
Source	Mouse
Concentration	~0.5mg/ml
Form	Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
Purity	Purified by Protein G affinity chromatography.
Endotoxin	<0.1EU/ug (LAL)
Immunogen	Recombinant corresponding to mouse 4-1BB Ligand extracellular domain expressed in Sf-21.
Specificity	Recognizes mouse 4-1BB Ligand. In direct ELISA, does not crossreact with recombinant human RELT.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.