4-1BB Ligand is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF9). It is expressed on activated antigen presenting cells (APCs) and on most T and B lymphoma cell lines. Soluble 4-1BB Ligand can be released from the cell surface following cellular activation via proteolytic cleavage. 4-1BB Ligand exists as a noncovalent homotrimer and interacts with 4-1BB (TNFRSF9) to provide T cell costimulatory signals and dendritic cell activation.
Applications
Suitable for use in Western Blot, Flow Cytometry and ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1-2ug/ml. Flow Cytometry: 0.25ug/ml. 10ul of the diluted solution labels 1x10e6 cells in a total reaction volume ≤ 200ul Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Arg104-Glu309 from mouse 4-1BB Ligand extracellular domain expressed in Sf-21.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant. Endotoxin: ≤0.1EU/ug (LAL)
Specificity
Recognizes mouse 4-1BB Ligand. In direct ELISA, does not crossreact with recombinant human RELT.
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