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You are here:Home » Antibodies » Abs to CD Markers » Anti -4-1BB Receptor (CD137, 4-1BB Ligand Receptor, TNFRSF9, CDw137, ILA, MGC2172, T-cell antigen 4-1BB homolog) (PE)

Anti -4-1BB Receptor (CD137, 4-1BB Ligand Receptor, TNFRSF9, CDw137, ILA, MGC2172, T-cell
antigen 4-1BB homolog) (PE)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified FC
4-1BB is an inducible T cell surface protein belonging to the TNF receptor superfamily. It is alternatively known as TNFRSF9, CD137, and ILA. The 255aa human 4-1BB is a type I transmembrane protein having in its extracellular domain four of the cysteine-rich motifs that are characteristic of the TNF receptor superfamily. The 30 KD glycoprotein exists both as a
monomer and as a dimer on T cells. The human and mouse proteins share 60% aa identity. 4-1BB is absent from naive T cells, but it is upregulated and continually expressed following T cell activation. The natural ligand, 4-1BBL, is a member of the TNF superfamily and is expressed on activated antigen presenting cells including dendritic cells, macrophages, and B cells. Cross-
linking of 4-1BB by 4-1BBL or by agonistic antibodies transmits a potent co-stimulatory signal that enhances the effect of other activating signals such as PHA or anti-CD3 antibodies. 4-1BB signals through the TFAF2-NIK pathway resulting in activation of NF- B and ultimately promoting proliferation and survival of T cells. For recent reviews on 4-1BB see Vinay, D. and B. Kwon,
1998, Semin. Immunol. 10:481 and Sica, G. and L. Chen, 2000 Adv. Exp. Med. Biol. 465:355.
Catalog #0004-01L
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionFlow Cytometry: Designed to quantitatively determine the percentage of cells bearing 4-1BB Ligand/TNFSF9 within a population and qualitatively determine the density of 4-1BB Ligand/TNFSF9 on cell surfaces by flow cytometry. Washed cells are incubated with the phycoerythrin-labeled monoclonal antibody, which binds to cells expressing 4-1BB Ligand/TNFSF9. Unbound phycoerythrin-conjugated antibody is then washed from the cells. Cells expressing 4-1BB Ligand/TNFSF9 are fluorescently stained, with the intensity of staining directly proportional to the density of expression of 4-1BB Ligand/TNFSF9. Cell surface expression of 4-1BB Ligand/TNFSF9 is determined by flow cytometric analysis using 488nm wavelength laser excitation and monitoring emitted fluorescence with a detector optimized to
collect peak emissions at 565-605nm.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No9H3
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in 50 ug of antibody in 1 mL PBS, 0.1% sodium azide.
PurityPurified
ImmunogenSynthetic peptide corresponding to human 4-1BB/TNFRSF9 (PE).
SpecificityRecognizes human 4-1BB/TNFRSF9 (PE).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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