4-1 BB Ligand, Recombinant, Human, aa71-254, His-Tag (4-1BBL, CD137L, TNFSF9, Tumor
necrosis factor ligand superfamily member 9)
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| Human 4-1BB Ligand (4-1BBL, CD137L) is a 32kD type II transmembrane protein that belongs to TNF superfamily (TNFSF) of molecules.1-4 The human 4-1BBL cDNA encodes a 254 amino acid (aa) protein that contains a 25aa N-terminal cytoplasmic domain, a 23aa transmembrane segment, and a 206aa C-terminal extracellular region.5 The extracellular domain (ECD) of 4-1BBL has a jelly-roll, -sandwich tertiary structure that is similar to other TNFSF members. There is only one cysteine in the human ECD, and no potential N-linked glycosylation sites. The potential exists, however, for O-linked glycosylation. The human 4-1BBL ECD shares 32% and 35% aa identity with mouse and rat ECD, respectively. In the cytoplasmic domain, human 4-1BBL is 55aa shorter than the equivalent region in rodents. 4-1BBL is expressed by activated B cells, macrophages, dendritic cells, activated Tcells, neurons and astrocytes.2, 3, 6 A 26kD soluble form of 4-1BBL is known to occur in human. Although it is presumably generated by MMP activity, its amino acid size is currently unreported.4 The soluble form is bioactive. Human 4-1BBL signals through both CD137/4-1BB and itself. Its cytoplasmic tail participates in reverse signaling that induces apoptosis in T cells and cytokine secretion (IL-6; TNF- ) by monocytes.7, 8 4-1BBL binding to CD137/4-1BB produces a number of effects. Its seems to play a key role in the T cell recall response. It maintains T cell numbers at the end of a primary response, and induces CD4+ and CD8+ T cells to proliferate and secrete cytokines such as IL-2 and IFN- in CD4+ cells, and IFN- in CD8+ cells.9, 10 | | | Catalog # | 0004-01T | | Source | Recombinant human 4-1BB ligand, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. | | Molecular Weight | 23.8kD (222aa) | | Concentration | ~1mg/ml (determined by Bradford assay,15% SDS-PAGE (3ug) | | Sequence | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMRE GPELSPDDPA GLLDLRQGMF AQLVAQNVLL IDGPLSWYSD PGLAGVSLTG | | GLSYKEDTKE LVVAKAGVYY VFFQLELRRV VAGEGSGSVS LALHLQPLRS AAGAAALALT VDLPPASSEA RNSAFGFQGR LLHLSAGQRL | | GVHLHTEARA RHAWQLTQGA TVLGLFRVTP EIPAGLPSPR SE | | Storage and Stability | Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. | | Source | Recombinant, human from E. coli | | Purity | 95%, as determined by SDS-PAGE and visualized by silver stain. | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 20mM Tris-HCl, pH 8, 100mM sodium chloride, 20% glycerol. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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