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Abs to Translation Initiation Factors
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Anti -4E Binding Protein 1 (Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP)
Anti -4E Binding Protein 1 (Protein Synthesis Initiation Factor 4E Binding Protein,
eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Purified |
B IH FC |
|
| Catalog # | 0004-18D | | Applications | Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Flow Cytometry: 1:20 stains cells for intracellular staining. | | Western Blot: 1:1000 detects endogenous 4E-BP1 in lysates from K562 cells. | | Immunoprecipitation: 1:50 immunoprecipitates 4E-BP1 using protein G:agarose beads. | | Immunohistochemistry: 1:50-1:100. Paraffin-embedded human prostate carcinoma using the anti-4E-BP1.Lysate from K562 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-4E-BP1 (1:1,000). Proteins were visualized using goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 7i3 | | Host | Rabbit | | Concentration | As reported | | Form | Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M NaCl, 0.01% sodium azide, 0.05% BSA), 40% glycerol. | | Purity | Purified | | Immunogen | Synthetic peptide corresponding to the C-terminal region of human 4E-BP1 (eIF4E-binding protein). | | Specificity | Recognizes the C-terminal domain of 4E-BP1. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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