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You are here:Home » Antibodies » Abs to Translation Initiation Factors » Anti -4E Binding Protein 1 (Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP)

Anti -4E Binding Protein 1 (Protein Synthesis Initiation Factor 4E Binding Protein,
eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Purified B IH FC
Catalog #0004-18D
ApplicationsSuitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:20 stains cells for intracellular staining.
Western Blot: 1:1000 detects endogenous 4E-BP1 in lysates from K562 cells.
Immunoprecipitation: 1:50 immunoprecipitates 4E-BP1 using protein G:agarose beads.
Immunohistochemistry: 1:50-1:100. Paraffin-embedded human prostate carcinoma using the anti-4E-BP1.Lysate from K562 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-4E-BP1 (1:1,000). Proteins were visualized using goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No7i3
HostRabbit
Concentration~1mg/ml
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M NaCl, 0.01% sodium azide, 0.05% BSA), 40% glycerol.
PurityPurified
ImmunogenSynthetic peptide corresponding to the C-terminal region of human 4E-BP1 (eIF4E-binding protein).
SpecificityRecognizes the C-terminal domain of 4E-BP1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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