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You are here:Home » Antibodies » Abs to Translation Initiation Factors » Anti -4E Binding Protein 1,2,3 phosphorylated (Thr45) (4E-BP123, 4EBP1, 4EBP2, 4EBP3, 4E-BP1, Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP))

Anti -4E Binding Protein 1,2,3 phosphorylated (Thr45) (4E-BP123, 4EBP1, 4EBP2, 4EBP3,
4E-BP1, Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I,
Eukaryotic Translation Initiation Factor BP))

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IC
4E-BP1 is a eukaryotic translation initiation factor (eIF4E- binding protein), also known as PHAS-1. The binding of 4E-BP to eIF4E is regulated by phosphorylation and is reversible. Non-phosphorylated 4E-BP1 bind strongly to eIF4E; however, phosphorylated 4E-BP1 will not bind to eIF4E. Akt, TOR, MAP, S6, and Cdc2 kinases are able to inactivate 4E-BP1 by phosphorylation. 4E-BP1 undergoes phosphorylation which releases it from eIF4E, ultimately forming initiation complexes that aid in the upregulation of protein synthesis.
Catalog #0004-19J
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:5,000-10,000
Immunohistochemistry (paraffin): 1:50-100
Immunocytochemistry: 1:100-250
Immunoprecipitation: 1:10
Optimal dilutions to be determined by the researcher.
Positive ControlUntreated and FBS-treated 293T cell lysate.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No9i141
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
PuritySupernatant
ImmunogenPhospho-specific peptide corresponding to residues surrounding Thr45 of human 4E-BP1/2/3.
SpecificityRecognizes 4E-BP1/2/3 phosophorylated at Thr45.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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