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You are here:Home » Antibodies » Abs to Translation Initiation Factors » Anti -4E-BP1, phosphorylated (Ser65) (4EBP1, 4E-BP1, Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP)

Anti -4E-BP1, phosphorylated (Ser65) (4EBP1, 4E-BP1, Protein Synthesis Initiation Factor
4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor
BP)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B
E-BP1 is a eukaryotic translation initiation factor (eIF4E- binding protein), also known as PHAS-1. The binding of 4E-BP to eIF4E is regulated by phosphorylation and is reversible. Non-phosphorylated 4E-BP1 bind strongly to eIF4E; however, phosphorylated 4E-BP1 will not bind to eIF4E. Akt, TOR, MAP, S6, and Cdc2 kinases are able to inactivate 4E-BP1 by phosphorylation. 4E-BP1 undergoes phosphorylation which releases it from eIF4E, ultimately forming initiation complexes that aid in the upregulation of protein synthesis.
Catalog #0004-19L
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:2000 detected 4E-BP1 in NIH/3T3 cell lysate
Optimal dilutions to be determined by the researcher.
Positive ControlNIH/3T3 cell lysates
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No10F618
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
PuritySupernatant
ImmunogenPhospho-specific peptide corresponding to residues surrounding Ser65 of human 4E-BP1.
SpecificityRecognizes human 4E-BP1 phosphorylated at Ser65. MW: ~20kD
Species CrossreactivityMouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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