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You are here:Home » Antibodies » Abs to Translation Initiation Factors » Anti -4E Binding Protein 1, phosphorylated (Thr70) (Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP)

Anti -4E Binding Protein 1, phosphorylated (Thr70) (Protein Synthesis Initiation Factor 4E
Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
4E-BP1 (also known as PHAS-1) normally binds eIF4E, inhibiting cap-dependent translation. Hyperphosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. The PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1. 4E-BP1 is phosphorylated in vivo on multiple residues; phosphorylation by FRAP/mTOR on Thr37 and Thr46 of human 4E-BP1 may prime it for subsequent phosphorylation at sites including Ser65 and Thr70.
Catalog #0004-20A
ApplicationsSuitable for use in Western Blot, and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blotting: 1:1000 (Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.)
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Molecular Weight 15-20kD
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
ConcentrationNot determined
FormSupplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA 50% glycerol. No preservative added.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic phosphopeptide derived from the sequence of mouse 4E-BP1.
SpecificityDetects endogenous levels of 4E-BP1 only when phosphorylated at Thr70. Does not detect 4E-BP1 phosphorylated at other sites. Species Cross-Reactivity: human, mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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