Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Translation Initiation Factors » Anti -4E Binding Protein 1, non-phosphorylated (Thr46) (Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP)

Anti -4E Binding Protein 1, non-phosphorylated (Thr46) (Protein Synthesis Initiation
Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation
Factor BP)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant E B FC
4E-BP1 (also known as PHAS-1) when bound to eIF4E, inhibits cap-dependent translation. Upon hyperphosphorylation of 4E-BP1 this interaction is disrupted and cap-dependent translation is activated (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4); while phosphorylation by FRAP/mTOR on Thr37 and Thr46 does not prevent the binding of 4E-BP-1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).
Catalog #0004-20B
ApplicationsSuitable for use in Flow Cytometry, ELISA, Western Blot. Other applications not tested.
Recommended DilutionWestern blot 1:1000
Flow Cytometry 1:200
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No7H1
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES (pH 7.5), 150mM NaCl, 100ug/ml BSA, 50% glycerol and less than 0.02% sodium azide.
PuritySupernatant
ImmunogenSynthetic peptide (KLH-coupled) corresponding to residues surrounding Thr46 of human 4E-BP1.
SpecificityDetects endogenous levels of 4E-BP1 only when dephosphorylated at Thr46. Crossreacts with 4E-BP2 and 4E-BP3 dephosphorylated at equivalent sites.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links