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You are here:Home » Antibodies » Abs to Translation Initiation Factors » Anti -4E Binding Protein 1 (Eukaryotic Translation Initiation Factor 4E Binding Protein 1, eIF4E Binding Protein 1, eIF4EBP1, 4EBP1, 4E-BP1, BP-1, MGC4316, Phosphorylated Heat- and Acid-stable Protein Regulated by Insulin 1, PHAS-1, PHAS I, PHAS-I)

Anti -4E Binding Protein 1 (Eukaryotic Translation Initiation Factor 4E Binding Protein 1,
eIF4E Binding Protein 1, eIF4EBP1, 4EBP1, 4E-BP1, BP-1, MGC4316, Phosphorylated Heat- and
Acid-stable Protein Regulated by Insulin 1, PHAS-1, PHAS I, PHAS-I)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH FC IF
Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the eIF4E translation initiation factor. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR on Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).
Catalog #0004-21A
ApplicationsSuitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunofluorescence (IF-IC): 1:400
Flow Cytometry: 1:200
Western Blot: 1:1000
Immunoprecipitation: 1:50
Immunohistochemistry (Paraffin): 1:600
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No10g124
HostRabbit
ConcentrationNot determined.
FormSupplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, < 0.02% sodium azide, 50% glycerol.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to residues surrounding Ser112 of human 4E-BP1.
SpecificityRecognizes endogenous levels of human total 4E-BP1 protein. Species Crossreactivity: monkey, mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesProtein Synthesis Initiation Factor 4E Binding Protein


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