Anti -4E Binding Protein 1 (Eukaryotic Translation Initiation Factor 4E Binding Protein 1,
eIF4E Binding Protein 1, eIF4EBP1, 4EBP1, 4E-BP1, BP-1, MGC4316, Phosphorylated Heat- and
Acid-stable Protein Regulated by Insulin 1, PHAS-1, PHAS I, PHAS-I)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
B IP IH FC IF |
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| Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the eIF4E translation initiation factor. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR on Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5). | | | Catalog # | 0004-21A | | Applications | Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Immunofluorescence (IF-IC): 1:400 | | Flow Cytometry: 1:200 | | Western Blot: 1:1000 | | Immunoprecipitation: 1:50 | | Immunohistochemistry (Paraffin): 1:600 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 10g124 | | Host | Rabbit | | Concentration | Not determined. | | Form | Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, < 0.02% sodium azide, 50% glycerol. | | Purity | Supernatant | | Immunogen | Synthetic peptide corresponding to residues surrounding Ser112 of human 4E-BP1. | | Specificity | Recognizes endogenous levels of human total 4E-BP1 protein. Species Crossreactivity: monkey, mouse, rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | Protein Synthesis Initiation Factor 4E Binding Protein |
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Download Specifications for Anti -4E Binding Protein 1 (Eukaryotic Translation Initiation Factor 4E Binding Protein 1, eIF4E Binding Protein 1, eIF4EBP1, 4EBP1, 4E-BP1, BP-1, MGC4316, Phosphorylated Heat- and Acid-stable Protein Regulated by Insulin 1, PHAS-1, PHAS I, PHAS-I) |
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