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You are here:Home » Antibodies » Abs to Translation Initiation Factors » Anti -EIF4EBP1, phosphorylated (Thr45) (Eukaryotic Translation Initiation Factor 4E-binding Protein 1, 4E-BP1, eIF4E-binding Protein 1, Phosphorylated Heat- and Acid-stable Protein Regulated by Insulin 1, PHAS-I)

Anti -EIF4EBP1, phosphorylated (Thr45) (Eukaryotic Translation Initiation Factor
4E-binding Protein 1, 4E-BP1, eIF4E-binding Protein 1, Phosphorylated Heat- and
Acid-stable Protein Regulated by Insulin 1, PHAS-I)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
4E-BP1 is a eukaryotic translation initiation factor (eIF4E- binding protein), also known as PHAS-1. The binding of 4E-BP to eIF4E is regulated by phosphorylation and is reversible. Non-phosphorylated 4E-BP1 bind strongly to eIF4E; however, phosphorylated 4E-BP1 will not bind to eIF4E. Akt, TOR, MAP, S6, and Cdc2 kinases are able to inactivate 4E-BP1 by phosphorylation. 4E-BP1 undergoes phosphorylation which releases it from eIF4E, ultimately forming initiation complexes that aid in the upregulation of protein synthesis.
Catalog #0004-21L
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot:1:500-1:1,000
Immunohistochemistry:1:50-1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to human 4E-BP1 around the phosphorylation site of threonine 45 (S-T-TP-P-G).
SpecificityRecognizes human 4EBP1 when phosphorylated at (Thr45). May cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. Species Cross-Reactivity: mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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