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You are here:Home » Antibodies » Abs to DNA/RNA Translation » Anti -4E Binding Protein 2 (4E-BP2, eIF4E-BP2, PHAS-2)

Anti -4E Binding Protein 2 (4E-BP2, eIF4E-BP2, PHAS-2)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
PHAS-II, also known as eIF4E-binding protein II (eIF4E-BPII), is a member of a family of proteins which regulate initiation (1). PHAS-I and ñII were found to have overlapping but different patterns of expression in tissues. PHAS-II shows the highest expression in liver and kidney where very little PHAS-I is found (2). The PHAS proteins regulate translation initiation by binding to the inhibitory protein eIF-4E and blocking translation by preventing access of eIF-4G to the 5í cap of the mRNA (1). Both PHAS proteins are phosphorylated in response to insulin or growth factors such as EGF, PDGF and IGF-1. Phosphorylation in the appropriate site(s) promotes dissociation of PHAS/eIF-4E complexes which allows eIF-4E to bind eIF-4G(p220), thereby increasing the amount of eIF-4F complex and the rate of translation initiation (1). Regulation of the two protein differ because PHAS-II, unlike PHAS-I is readily phosphorylated by PKA in vitro. However increasing cAMP in cells promotes dephosphorylation of both PHAS-I and PHAS-II. Pharmacological and genetic evidence indicates that the mTOR/p70S6K pathway is involved in the control of PHAS-I and ñII suggesting that these proteins may be mediators of the effects of this pathway on protein synthesis and cell proliferation (1).
Applications:
Western Blot (Colorimetric): 4ug/ml
Western Blot (ECL): 4ug/ml
Optimal dilutions to be determined by researcher.
Positive Controls:
Rat Brain Tissue Extract
Catalog #0004-30
Clone TypePolyclonal
HostRabbit
SourceHuman
FormSupplied as a liquid in borate buffered saline, pH 8.4 25mM sodium borate, 100mM boric acid, 75mM sodium chloride, 5mM EDTA (disodium salt).
PurityPurified by immunoaffinity chromatography.
ImmunogenHuman PHAS-II synthetic peptide (residues 99-120) was synthesized and the peptide coupled to KLH. This sequence is 96% homologous to mouse PHAS-II over these residues.
SpecificityThis immunoaffinity purified antibody detects 17 and 20kD proteins, corresponding to the apparent molecular mass of PHAS-II and its phosphorylated state on SDS-PAGE immunoblots, in samples from human, mouse, rat, canine, porcine, rabbit (weakly) and bovine. Does not react with recombinant rat PHAS-I.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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