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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B

14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling. The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders. 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms. The 14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking. These functions of 14-3-3 proteins are facilitated by, if not dependent on, its dimeric structure. Recent work has demonstrated that the dimeric status of the 14-3-3 protein is regulated by site-specific serine phosphorylation. (1-5)
Catalog #	0014-01B
Applications	Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 1:1,000. Detects a band of ~ 29kD.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Source	Rat
Concentration	Not determined
Form	Supplied as a liquid in 10 mM Hepes, pH7.5, containing 150 mM NaCl, 100 ug/ml BSA and 50% glycerol.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser58 of rat 14-3-3 protein.
Specificity	Recognizes 14-3-3 protein. Species Crossreactivity: human, mouse, Xenopus, and rat based on 100% antigen sequence homology.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names	Protein kinase C inhibitor protein 1, KCIP-1, KCIP 1, Protein 1054