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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B

14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders (Berg et al., 2003). 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms (Silhan et al., 2004; Dougherty and Morrison, 2004). The14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking. These functions of 14-3-3 proteins are facilitated by, if not dependent on, its dimeric structure. Recent work has demonstrated that the dimeric status of the 14-3-3 protein is regulated by site-specific serine phosphorylation (Woodcock et al., 2003).
Catalog #	0014-01Q
Applications	Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 1:1000
	Optimal dilutions to be determined by the researcher.
Positive Control	Rat brainstem lysate
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Rat
Concentration	Not determined
Form	Supplied as a liquid 10mM HEPES, pH 7.5, 150mM sodium chloride, 100ug per ml BSA, 50% glycerol.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthetic peptide corresponding to amino acid residues surrounding the phosphorylated (Ser58) of rat 14-3-3.
Specificity	Recognizes rat 14-3-3, phosphorylated (Ser58). Species Sequence Homology: human and mouse.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.