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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IP

14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14-3-3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein.
Catalog #	0014-01X
Applications	Suitable for use in Immunoprecipitation and Western Blot. Other applications have not been tested.
Recommended Dilutions	Immunoprecipitation (Denatured): 2ug/mg protein lysate using Protein G.
	Western Blot: 1-2ug/ml for 2 hrs at RT
	Optimal dilutions to be determined by the researcher.
Positive Control	HeLa cells
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	3F7
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in 10mM PBS, pH 7.4. No stabilizing proteins and no preservatives added. Also available with BSA and azide. See 0014-01.
Purity	Purified by Protein G affinity chromatography.
Immunogen	14-3-3 gamma recombinant protein of human origin (26-33kD) Cellular Localization: Cytoplasmic
Specificity	Recognizes human beta, gamma, sigma and zeta isoforms of 14-3-3.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.