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You are here:Home » Antibodies » Abs to 14-3-3 » Anti -14-3-3, pan (BSA & Azide Free)

Anti -14-3-3, pan (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP
14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14-3-3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein.
Catalog #0014-01X
ApplicationsSuitable for use in Immunoprecipitation and Western Blot. Other applications have not been tested.
Recommended DilutionsImmunoprecipitation (Denatured): 2ug/mg protein lysate using Protein G.
Western Blot: 1-2ug/ml for 2 hours at RT
Optimal dilutions to be determined by the researcher.
Positive ControlHeLa cells
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No3F7
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4. No stabilizing proteins and no preservatives added. Also available with BSA and azide. See 0014-01.
PurityPurified by Protein G affinity chromatography.
Immunogen14-3-3 gamma recombinant protein of human origin (26-33kD) Cellular Localization: Cytoplasmic
SpecificityRecognizes human beta, gamma, sigma and zeta isoforms of 14-3-3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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