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Abs to 14-3-3
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Anti -14-3-3, beta (Tyrosine 3-monooxygenase/tryptopan 5-monooxygenase activation protein, beta polypeptide)
Anti -14-3-3, beta (Tyrosine 3-monooxygenase/tryptopan 5-monooxygenase activation protein,
beta polypeptide)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IP |
|
| At least 7 mammalian isoforms comprise the 14-3-3 family of signaling intermediates. 14-3-3 moleculaes are highly conserved, expressed in a broad range of tissues and cell types and were first discovered as a series of highly abundant proteins in brain tissue. There is increasing evidence that 14-3-3 proteins are critical to cell transformation and mitotic signaling. | | | Catalog # | 0014-02A | | Applications | Suitable for use in Western Blot and Immunoprecipitation. | | Other applications not tested. | | Recommended Dilution | Western Blot; 2-4ug/ml for 2 hrs at RT | | Optimal dilutions to be determined by researcher | | Positive Control | HT29 cells, NIH/3T3 cells | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Clone Type | Polyclonal | | Host | Rabbit | | Source | Human | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.6, 0.2% BSA and 15mM sodium azide, before the addition of glycerol to 40%. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | A synthetic peptide from N-terminus of human 14-3-3 beta. Cellular Localization | | Specificity | Recognizes human 14-3-3 beta. Species Crossreactivity: Mouse and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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