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You are here:Home » Antibodies » Abs to 14-3-3 » Anti -14-3-3 beta (Tyrosine 3-monooxygenase/tryptopan 5-monooxygenase Activation Protein beta Polypeptide, YWHAB)

Anti -14-3-3 beta (Tyrosine 3-monooxygenase/tryptopan 5-monooxygenase Activation Protein
beta Polypeptide, YWHAB)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH IC IF
This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene.
Catalog #0014-02P2
ApplicationsSuitable for use in Western Blot, Immunocytochemistry, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:5000-1:20,000
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
Immunofluorescence: 1:100-1:200
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7, 0.01% thimerosal, 10% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to aa34-221 of human 14-3-3 beta.
SpecificityRecognizes human YWHAB. Species sequence homology: mouse (84%), rat (97%) and zebrafish (82%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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